Searching for potent and specific antibiotics against pathogenic Helicobacter and Campylobacter strains
Polyunsaturated fatty acids (PUFAs) and siamycin
Matsui et al. screened for specific inhibitors of the futalosine pathway in culture broth samples of 6183 microbes including 2160 fungi, 3783 actinomycetes, and 240 lactobacilli. They employed a paper disk method using two indicator microorganisms, B. halodurans C-125 (the futalosine pathway) and B. subtilis H17 (the canonical pathway). Isolation and structural elucidation of active compounds from screening hits revealed that ω-3 and ω-6 PUFAs, including α-linoleic acid, γ-linoleic acid, arachidonic acid, eicosapentaenoic acid (EPA, 20), and docosahexaenoic acid (DHA, 21), as well as, siamycin I (22), a lasso peptide natural product, specifically inhibited the futalosine pathway . They next showed that compounds 20–22 suppressed the growth of H. pylori strains SS1 and TN2GF4 in a dose-dependent manner. They also evaluated the effect of these compounds using a mouse model of H. pyloriinfection. Treatment of mice with EPA (30 μg/ml), DHA (30 μg/ml), or siamycin I (5 μg/ml) in drinking water significantly reduced the colonization of H. pylori by 96, 78, and 68%, respectively. In addition, they prepared 10-hydroxy-cis-12-octadecenoic acid (HYA; C18:1 ω-6, 23) from linoleic acid by bio-conversion with Lactobacillus plantarum and showed that 23 also suppressed the in vitro growth and in vivo colonization of H. pylori by blocking the futalosine pathway. HYA was also effective against Helicobacter suis, which causes the formation of gastric lymphoid follicles , when 23 was added to the drinking water of a mouse model of H. suis infection.
ChooseLife Notes : EPA reduced H.pylori colonisation by 96% (which the body can readily create from ALA from Flax or absorb from Fish Liver Oil).