{"id":1517,"date":"2020-02-26T11:40:41","date_gmt":"2020-02-26T11:40:41","guid":{"rendered":"http:\/\/chooselife.co.uk\/?p=1517"},"modified":"2020-02-26T11:42:44","modified_gmt":"2020-02-26T11:42:44","slug":"blueberry-powder-breast-tumors","status":"publish","type":"post","link":"https:\/\/chooselife.co.uk\/index.php\/2020\/02\/26\/blueberry-powder-breast-tumors\/","title":{"rendered":"Whole blueberry powder modulates the growth and metastasis of MDA-MB-231 triple negative breast tumors in nude mice."},"content":{"rendered":"\n

Authors – Adams LS,  Kanaya N,  Phung S,  Liu Z,  Chen S. <\/p>\n\n\n\n

Abstract<\/h3>\n\n\n\n

Previous studies in our laboratory demonstrated that blueberry (BB) extract exhibited antitumor activity against MDA-MB-231 triple negative breast cancer (TNBC) cells and decreased metastatic potential in vitro. <\/p>\n\n\n\n

The current study tested 2 doses of whole BB powder, 5 and 10% (wt:wt) in the diet, against MDA-MB-231 tumor growth in female nude mice. In this study, tumor volume was 75% lower in mice fed the 5% BB diet and 60% lower in mice fed the 10% BB diet than in control mice (P \u2264 0.05). <\/p>\n\n\n\n

Tumor cell proliferation (Ki-67) was lower in the 5 and 10% BB-fed mice and cell death (Caspase 3) was greater in the 10% BB-fed mice compared to control mice (P \u2264 0.05). <\/p>\n\n\n\n

Gene analysis of tumor tissues from the 5% BB-fed mice revealed significantly altered expression of genes important to inflammation, cancer, and metastasis, specifically, Wnt signaling, thrombospondin-2, IL-13, and IFN\u03b3. To confirm effects on Wnt signaling, analysis of tumor tissues from 5% BB-fed mice revealed lower \u03b2-catenin expression and glycogen synthase kinase-3\u03b2 phosphorylation with greater expression of the \u03b2-catenin inhibitory protein adenomatous polyposis coli compared to controls. <\/p>\n\n\n\n

A second study tested the ability of the 5% BB diet to inhibit MDA-MB-231-luc-D3H2LN metastasis in vivo. In this study, 5% BB-fed mice developed 70% fewer liver metastases (P = 0.04) and 25% fewer lymph node metastases (P = 0.09) compared to control mice. <\/p>\n\n\n\n

This study demonstrates the oral antitumor and metastasis activity of whole BB powder against TNBC in mice.<\/p>\n\n\n\n

\"\"
MDA-MB-231 tumor volume (A<\/em>), proliferation (B<\/em>), and apoptosis (C<\/em>) in female nude mice fed control, 5% BB powder, or 10% BB powder diet for 8 wk. Data are means \u00b1 SEM, n<\/em> = 6. In A<\/em>, labeled means at a time without a common letter differ, P<\/em> \u2264 0.05. In B<\/em> and C<\/em>, asterisks indicate different from control: *P<\/em> \u2264 0.05, **P<\/em> \u2264 0.01. BB, blueberry. <\/figcaption><\/figure>\n\n\n\n
\"\"
In situ tumor growth and metastasis monitored by Xenogen IVIS imaging at different time points after MDA-MB-231 tumor implantation in female nude mice fed control or 5% BB diet for 7 wk. (A<\/em>) Orthotopic breast tumor growth from baseline to wk 5 postimplantation. (B<\/em>) In situ and ex-vivo imaging of RLN and LLN lymph node metastasis at wk 5 postimplantation. (C<\/em>) Frequency of metastasis and mean intensity of liver and LN metastases analyzed by contingency table. Intensity data are mean \u00b1 SEM for the number of mice with metastases. *Different from control, P<\/em> \u2264 0.05. BB, blueberry; LLN, left lymph node; RLN, right lymph node. <\/figcaption><\/figure>\n\n\n\n

Source : https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21880954<\/a> <\/p>\n","protected":false},"excerpt":{"rendered":"

Authors – Adams LS,  Kanaya N,  Phung S,  Liu Z,  Chen S. Abstract Previous studies in our laboratory demonstrated that blueberry (BB) extract exhibited antitumor activity against MDA-MB-231 triple negative breast cancer (TNBC) cells and decreased metastatic potential in vitro. The current study tested 2 doses of whole BB powder, 5 and 10% (wt:wt) in … <\/p>\n

Continue reading “Whole blueberry powder modulates the growth and metastasis of MDA-MB-231 triple negative breast tumors in nude mice.”<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[24,35],"tags":[],"_links":{"self":[{"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/posts\/1517"}],"collection":[{"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/comments?post=1517"}],"version-history":[{"count":2,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/posts\/1517\/revisions"}],"predecessor-version":[{"id":1522,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/posts\/1517\/revisions\/1522"}],"wp:attachment":[{"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/media?parent=1517"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/categories?post=1517"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/chooselife.co.uk\/index.php\/wp-json\/wp\/v2\/tags?post=1517"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}