More good fats CLA C9-T11

Induction of apoptosis by c9, t11-CLA in human endometrial cancer RL 95-2 cells via ERα-mediated pathway.

Abstract

Numerous studies have shown that conjugated linoleic acid (CLA) can inhibit cancer cells growth and induce apoptosis in vitro and in vivo. The aim of the present study was to investigate the effects of CLA, including cis9, trans11-conjugated linoleic acid (c9, t11-CLA) and trans10, cis12-conjugated linoleic acid (t10, c12-CLA), on apoptosis of human endometrial cancer RL 95-2 cells and its related mechanisms. The MTT analysis was used to evaluate the effect of CLA isomers on the viability of endometrial cancer RL 95-2 cells. We then estimated the apoptosis by Morphological observation and Annexin V-FITC/PI staining and flow cytometry. We also used Western blot analysis to assess the expression of caspase-3, Bax, Bcl-2 proteins and the activation of Akt/p-Akt and ERα/p-ERα. Propylpyrazole-triol (PPT), a selective ERα agonist was used to confirm the induction of apoptosis by c9, t11 CLA may relate to ERα-mediated pathway. In CLA-treated RL 95-2 cells, we found that c9, t11-CLA inhibited viability and trigged apoptosis, as judged from nuclear morphology and flow cytometric analysis. The expression of caspase-3 and the ratio of Bax/Bcl-2 were significant increased, but no obvious change was observed about Akt and p-Akt in c9, t11-CLA-treated cells. However, the expression of total ERα level in RL 95-2 cells-treated with c9, t11-CLA was unchanged, while in the concentration of 80 mM, c9, t11-CLA down-regulated the protein expression level of p-ERα. Then PPT has the antagonistic action on growth inhibitory effect in RL 95-2 cells incubated with c9, t11-CLA. This study demonstrated that c9, t11- CLA could induce apoptosis in RL 95-2 cells, and may involve in ERα-mediated pathway. These results indicated that c9, t11- CLA could induce apoptosis of endometrial cancer cells and may be potential agents for the treatment of endometrial cancer.

KEYWORDS:

Apoptosis; Conjugated linoleic acid; Endometrial cancer; Estrogen receptor

PMID: 23954748

Choose Life Notes : When my daughter was born extremely prematurely, I was aware of this acid from studies showing it is found in around 50% higher  amounts in mothers breast milk who are eating (almost) exclusively organic vs non-organic,  women’s breast milk was tested and it showed that their milk was also many fold richer in this heavyweight nutrient. We had grass fed raw Milk (knowing this also increased the amounts by potentially 300-500%) and the Weston Price based formula of High Vitamin Butter Oil and Fermented Cod Liver Oil (not personally as vegetarian, but I encouraged my wife daily to take this through pregnancy and the lactation period).

This formula:

 

This formula, which I believe Weston Price referred to as ActivatorX, bears striking similarity to Johanna Budwigs Flax and Cottage Cheese blend, both are 2-1 Omega 6:3.

This research shows that CLA C9 T11 was found in the highest amounts in short fermented organic milk:

Abstract
This study investigates the kinetics of acidification, fatty acid (FA) profile and conjugated linoleic acid (CLA, C18:2 c9, t11) content in fermented milks prepared from organic and conventional milk. Fermented milks were manufactured with five mixed cultures: four different strains of Bifidobacterium animalis subsp. lactis (BL04, B94, BB12 and HN019) and Lactobacillus delbrueckii subsp. bulgaricus LB340, in co-culture with Streptococcus thermophilus TA040. The composition of milk was evaluated, and the kinetics of acidification was followed by continuous pH measurement using the Cinac system. The profile of FA, including CLA, was analyzed by gas chromatography. The chemical composition of conventional and organic milk was similar, with the exception of protein and Fe, the concentrations of which were higher in the organic milk. The rate of acidification was significantly influenced by the type of milk and the bacterial strain used. Co-cultures St-HN019 and St-BB12 showed higher maximal acidification rates in both milks. Final counts of S. thermophilus (9.0-10.1 log10 colony forming units (CFU)·mL-1), Lactobacillus bulgaricus (8.2-8.5 log 10 CFU·mL-1) and B. animalis subsp. lactis strains (8.3-9.3 log10 CFU·mL-1) did not differ significantly in either milk.
Unexpectedly, all fermented organic milks contained significantly higher amounts of CLA than the same milk before fermentation, whereas CLA amounts did not change during fermentation of conventional milk. Regardless of the type of milk, CLA was found to be significantly positively correlated with trans-vaccenic acid and negatively correlated with linoleic acid. Moreover, the CLA contents were significantly higher in fermented milks showing shorter fermentation times.

Increased CLA content in organic milk fermented by bifidobacteria or yoghurt cultures | Request PDF. Available from: https://www.researchgate.net/publication/44844024_Increased_CLA_content_in_organic_milk_fermented_by_bifidobacteria_or_yoghurt_cultures [accessed Sep 14 2018].

Choose Life Notes : This highlights the potential importance of Grass Fed Raw Kefir as the Sulfur Protein element in the Budwig Protocol, also within the GC Maf protein mixtures, adopting a best attainable approach, we latterly used to get A2 Raw Milk from Hurdlebrook Farm, they are converting to Organic now, perhaps we will start buying from there again, and make some kefir, as my wife has just started working near by again, serendipitously.

More Studies:

Atheroprotective effects of conjugated linoleic acid.

Abstract

Atherosclerosis, the underlying cause of heart attack and strokes, is a progressive dyslipidaemic and inflammatory disease where monocyte-derived macrophage cells play a pivotal role. Although most of the mechanisms that contribute to the progression of atherosclerosis have been identified, there is limited information on those governing regression. Conjugated linoleic acid (CLA) is a generic term denoting a group of naturally occurring isomers of linoleic acid (18:2, n6) that differ in the position or geometry (i.e. cis or trans) of their double bonds. The most predominant isomers in ruminant fats are cis-9, trans-11 CLA (c9,t11-CLA), which accounts for more than 80% of CLA isomers in dairy products and trans-10, cis-12 CLA (t10,c12-CLA). Dietary administration of a blend of the two most abundant isomers of CLA has been shown to inhibit the progression and induce the regression of pre-established atherosclerosis. Studies investigating the mechanisms involved in CLA-induced atheroprotective effects are continually emerging. The purpose of this review is to discuss comprehensively the effects of CLA on monocyte/macrophage function in atherosclerosis and to identify possible mechanisms through which CLA mediates its atheroprotective effects.

KEYWORDS:

atherosclerosis; conjugated linoleic acid; inflammation; resolution.

 

Selective effect of conjugated linoleic acid isomers on atherosclerotic lesion development in apolipoprotein E knockout mice.

Abstract

Research suggests that conjugated linoleic acid (CLA) may inhibit atherosclerosis, but there are contradictory results in different animal models fed heterogeneous mixtures of CLA isomers. This study addressed the hypothesis that the individual CLA isomers may exert different atherogenic properties. ApoE(-/-) mice were fed isocaloric, isonitrogenous westernized diets containing 0.15% cholesterol and enriched with 1% (w/w) cis-9,trans-11-CLA (c9,t11-CLA), trans-10,cis-12-CLA (t10,c12-CLA) or linoleic acid (control diet) for 12 weeks. At the end of the dietary intervention, the effects of CLA isomers on the development of atherosclerotic vascular lesions, lipid metabolism, inflammation and oxidative stress were assessed. The t10,c12-CLA diet had a profound pro-atherogenic effect, whereas c9,t11-CLA impeded the development of atherosclerosis. En face aortic lesion assessment showed more dorsal and lumbar extensions presenting atherosclerotic foci after the t10,c12-CLA diet. Furthermore, animals fed t10,c12-CLA had pronounced hyperlipidemia, higher 8-iso-prostaglandin F(2alpha) levels, higher vulnerable atherosclerotic plaque with a lower smooth muscle and fibre contents and higher macrophage content and activation, assayed as plasma chitotriosidase compared to the control or c9,t11-CLA dietary groups. Plasma chitotriosidase activity was more closely associated with the extent of the plaque than with MOMA staining or than monocyte chemoattractant protein-1 levels.

Our results demonstrate that CLA isomers differentially modulate the development of atherosclerosis, c9,t11-CLA impedes, whereas t10,c12-CLA promotes atherosclerosis. These opposing effects may be ascribed to divergent effects on lipid, oxidative, inflammatory and fibro muscular components of this pathology. Plasma chitotriosidase is a better indicator of dietary fat interventions that alter plaque monocyte activity in this murine model.

c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway.

Abstract

Oxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the activation of nuclear factor E2-related factor-2 (Nrf2) and improved mitochondrial function, but less is known about the actions of specific isomers. The differential ability of individual CLA isomers to modulate these pathways was explored in Wistar rats fed for 4 weeks with a lard-based high-fat diet (L) or with control diet (CD), and, within each dietary treatment, two subgroups were daily administered with 9,11-CLA or 10,12-CLA (30 mg/day). The 9,11-CLA, but not 10,12-CLA, supplementation to CD rats improves the GSH/GSSG ratio in the liver, mitochondrial functions, and Nrf2 activity. Histological examination reveals a reduction of steatosis in L-fed rats supplemented with both CLA isomers, but 9,11-CLA downregulated plasma concentrations of proinflammatory markers, mitochondrial dysfunction, and oxidative stress markers in liver more efficiently than in 10,12-CLA treatment.

The present study demonstrates the higher protective effect of 9,11-CLA against diet-induced pro-oxidant and proinflammatory signs and suggests that these effects are determined, at least in part, by its ability to activate the Nrf2 pathway and to improve the mitochondrial functioning and biogenesis.

KEYWORDS:

fatty acids; mitochondrial efficiency; nuclear factor E2-related factor-2

PMID: 2463450

 

Infection and pH UTI

Urinary Tract Infection Natural Remedy: Low pH Levels In Urine And Certain Foods May Be To Blame For Frequent UTIs

Not every expert agrees cranberries, or cranberry juice, can help prevent urinary tract infections (UTIs): One study found people eating more cranberry products were 38 percent less likely to develop an infection, while a separate study found this benefit was small at best. However, the findings of a new study published in The Journal of Biological Chemistry may reconcile this difference of opinion.

UTIs typically occur when bacteria, such as E. coli, enter through the urinary tract and multiply in the bladder, the Mayo Clinic explained; though fungi and viruses can also cause a UTI. Once infected, the body will secrete a protein called siderocalin (SCN), which works as an antimicrobial to inhibit bacterial iron uptake. This uptake is how nearly all bacteria survive.

So to see how well SCN can influence an UTI, researchers sampled urine from volunteers previously participating in an immunological and pathogenic study conducted between 2008 and 2012. Among their results, they found urine better able to resist uptake had higher levels of pH (or more acid) and certain metabolites. Dr. Jeffrey P. Henderson, senior study author and assistant professor of medicine at Washington University School of Medicine in St. Louis, told Time the metabolites he and his team found were from dietary sources, not human cells.

“It looks like this protein that’s part of your immune system is able to use metabolites in the diet as grips to hold onto iron and keep it away from pathogenic bacteria,” Henderson said.

The high pH and metabolites together may be what promotes “restrictive urinary characteristics,” thus preventing or treating antibiotic-resistant E. coli UTI without compromising gut or vaginal microbes. More research would need to be done, but Henderson added the best part about his findings is the fact it proposes a non-antibiotic remedy.

Already now physicians recommend calcium-rich foods and supplements to boost urine pH. Think of citrus fruits, vegetables, and dairy products. But as Henderon points out to TIME, it’s harder to increase the levels of protective metabolites since there are individual differences to consider. Polyphenol-rich foods, however, are believed to source metabolities. These include tea, coffee, wine, and yep, cranberries.

Herein lies the possibility of reconciliation.

“One thing this suggests is that maybe the reason it’s not more effective is that people need both cranberries and a higher urine pH, or they need cranberries and appropriate inhabitants of their intestine, or the right microbiome composition in their gut, for the cranberry part to work properly,” Henderson concluded.

Source: Shields-Cutler, R.R., et al. Human Urinary Composition Controls Antibacterial Activity of Siderocalin. The Journal of Biological Chemistry. 2015.

ChooseLife : This is another signal that the terrain needs focus. We prefer Watermelon seed tea by Edgar Cayce (or just good Watermelon), along with alkalising techniques and taking any such issues as a sign dietary change is required. http://deepdownwellness.com/ddw/edgar-cayces-watermelon-seed-tea-for-kidney-stones/

Nutrient-Rich Diet May Help Heart Failure Patients Avoid Hospital, Death

Micronutrients in heart failure patients

TUESDAY, Sept. 4, 2018 (American Heart Association) — A varied, quality diet could help prevent hospitalizations and even death among patients with heart failure, a new study suggests.

Researchers investigating nutritional deficiencies found that people with heart failure who lack seven or more micronutrients had nearly double the risk of dying or being hospitalized than those who didn’t have any or only a few deficiencies. The University of Kentucky-led study was published Sept. 4 in the Journal of the American Heart Association.

“This establishes the importance of nutrition and why it really has to become a higher priority when it comes to treating heart failure,” said lead author Terry Lennie, senior associate dean at the University of Kentucky’s College of Nursing. “Nutritional deficiencies really can put patients at risk, more so than I think we understood or appreciated before.”

The study examined data from 246 patients recruited from three heart failure clinics in Georgia, Indiana and Kentucky. Patients kept detailed diaries of everything they ate and drank for four consecutive days.

Researchers assessed the intake of 17 micronutrients — 11 vitamins and six minerals — from the food diaries. They also kept tabs on patients every month for the following year.

The study found that 44 percent of patients with deficiencies in seven or more micronutrients were hospitalized or died within the year, compared to 25 percent of patients who had no deficiencies or only a few.

Calcium was the most commonly deficient micronutrient in patients’ diets, followed by magnesium, vitamins D and E, zinc and vitamin C.

One reason for the lack of these micronutrients could be “diet monotony,” or the tendency to eat the same foods every day instead of incorporating variety into meals. The study found many patients consumed the same foods for multiple meals across all four days of the food diary. Older adults are more vulnerable to this habit “due to a decreased drive to consume varied foods,” the study said. The average age of patients was 61.

A majority of the participants were overweight or obese, dispelling the notion of a link between a person’s weight and nutritional deficiencies.

“When we see individuals who are overweight, people tend to think they’re well-nourished, and that we only have to worry about people who are underweight as far as nutrition goes,” Lennie said. “But we found no relationship between patients’ body mass index and whether or not they had nutritional deficiencies.”

Dr. Frank Hu said the use of four-day food diaries did a good job capturing patient dietary patterns. But Hu, chairman of the nutrition department at Harvard University’s T.H. Chan School of Public Health, said he would have liked to have seen a much larger study size.

Hu, a professor of nutrition and epidemiology who was not involved in the research, said the findings demonstrate the role that well-rounded, varied diets can play in keeping heart failure patients alive. He noted the study did not address whether any single nutrient played a more important role than others. It instead looked at overall dietary health.

“It’s very important to pay attention to both nutrition quantity and quality. When we talk about nutrition quality, we’re not talking about just popping a vitamin or mineral supplement,” he said.

“Micronutrients come mostly from plant-based foods, such as fruits and vegetables, whole grains, nuts and legumes,” Hu said, adding that including some animal foods such as moderate amounts of fish and dairy products is also helpful in achieving adequate micronutrient intakes.

“It’s more important to pay attention to the quality of the foods when we try to make sure patients eat a balanced, nutritional meal,” he said.

Last Updated: 

Original article : https://consumer.healthday.com/vitamins-and-nutrition-information-27/food-and-nutrition-news-316/aha-nutrient-rich-diet-may-help-heart-failure-patients-avoid-hospital-death-737407.html

 

Seaweed reduces Methane production in Holstein Cows

https://phys.org/news/2018-08-seaweed-dairy-cows-emissions.html

University of California researchers are feeding seaweed to dairy cows in an attempt to make cattle more climate-friendly.

UC Davis is studying whether adding small amounts of seaweed to  can help reduce their emissions of methane, a  that’s released when cattle burp, pass gas or make manure.

In a study this past spring, researchers found  were reduced by more than 30 percent in a dozen Holstein cows that ate the ocean algae, which was mixed into their feed and sweetened with molasses to disguise the .

“I was extremely surprised when I saw the results,” said Ermias Kebreab, the UC Davis animal scientist who led the study. “I wasn’t expecting it to be that dramatic with a small amount of seaweed.”

Kebreab says his team plans to conduct a six-month study of a seaweed-infused diet in beef cattle starting in October.

More studies will be needed to determine its safety and efficacy, and seaweed growers would have to ramp up production to make it an economical option for farmers.

Dairy farms and other livestock operations are major sources of methane, a heat-trapping gas many times more potent than carbon dioxide.

ChooseLife : Seaweed and Molasses, is there no end to the magnificence of this combination? https://sicknesshope.com/ Moreless knew.

Thank You

In the past 2-3 years I have experienced a huge increase in personal sensitivity, especially related to emotional stimuli. This has led to understanding that I am a ‘Highly Sensitive Person’ (www.hsperson.com), or, ‘Innate Sensitive’ in Jungian Psychology .

During this process, and time, many times I become simply irate, after obvious courtesy is ignored (holding a door, letting a car through, basic social courtesy). Over the past few years I feel this had become more and more prevalent, believing it to be a localised issue firstly (that people in the new area we live, are more prone to social discourtesy), however I now believe that it may be a wider societal issue.

This new published study shows that courtesy matters, a lot:

Undervaluing Gratitude: Expressers Misunderstand the Consequences of Showing Appreciation.

Abstract

Expressing gratitude improves well-being for both expressers and recipients, but we suggest that an egocentric bias may lead expressers to systematically undervalue its positive impact on recipients in a way that could keep people from expressing gratitude more often in everyday life. Participants in three experiments wrote gratitude letters and then predicted how surprised, happy, and awkward recipients would feel. Recipients then reported how receiving an expression of gratitude actually made them feel. Expressers significantly underestimated how surprised recipients would be about why expressers were grateful, overestimated how awkward recipients would feel, and underestimated how positive recipients would feel. Expected awkwardness and mood were both correlated with participants’ willingness to express gratitude. Wise decisions are guided by an accurate assessment of the expected value of action. Underestimating the value of prosocial actions, such as expressing gratitude, may keep people from engaging in behavior that would maximize their own-and others’-well-being.

KEYWORDS:

gratitude; happiness; open data; open materials; preregistered; social cognition; social connection; well-being

PMID: 29949445

 

My wife always urges me to calm down about it, when I get upset, asking why it matters to me, if others are not polite, but I can’t help it, my high sensitivity makes me constantly look at the world and reflect how actions of people affect those around them.

Why would you ignore thanking someone? someone who has obviously been kind, gracious, or polite, to you? Happiness creates a positive thought form, we need more of this in our lives.

Thank you for reading this, and thank you for being thankful!

RIP Don Croft

The amount of positive energy, created by the drive of this soul, was staggering.

Some of the best years of my life have been spent gifting, along with Don and the rest of the community, though we never met, I am enriched as a being for reading his reflections.

So sorry to Carol and family for your loss.

Very Best Wishes, Rich

Body Clock Proteins, Macrophages and Moreless

Reading about new research results of a study into Inflammation and the body clock, here :

https://www.eurekalert.org/pub_releases/2018-08/r-nrr082018.php

Researchers at RCSI and Trinity College Dublin have revealed insights into how the body clock controls the inflammatory response, which may open up new therapeutic options to treat excess inflammation in conditions such as asthma, arthritis and cardiovascular disease. By understanding how the body clock controls the inflammatory response, we may be able to target these conditions at certain times of the day to have the most benefit. These findings may also shed light on why individuals who experience body clock disruption such as shift workers are more susceptible to these inflammatory conditions.

The body clock, the timing mechanism in each cell in the body, allows the body to anticipate and respond to the 24-hour external environment. Inflammation is normally a protective process that enables the body to clear infection or damage, however if left unchecked can lead to disease. The new study, led by researchers at Dr. Annie Curtis’s Lab at RCSI (Royal College of Surgeons in Ireland) in partnership with Prof. Luke O’Neill’s Lab at Trinity College Dublin, is published in the Proceedings of the National Academy of Sciences (PNAS), a leading international multidisciplinary scientific journal.

Dr Annie Curtis, Research Lecturer in the Department of Molecular and Cellular Therapeutics at RCSI and senior author, explained that: “Macrophages are key immune cells in our bodies which produce this inflammatory response when we are injured or ill. What has become clear in recent years is that these cells react differently depending on the time of day that they face an infection or damage, or when we disrupt the body clock within these cells”.

Dr. Jamie Early, first author on the study, said: “We have made a number of discoveries into the impact of the body clock in macrophages on inflammatory diseases such as asthma and multiple sclerosis. However, the underlying molecular mechanisms by which the body clock precisely controls the inflammatory response were still unclear. Our study shows that the central clock protein, BMAL1 regulates levels of the antioxidant response protein NRF2 to control a key inflammatory molecule called IL-1β from macrophages.”

“The findings although at a preliminary stage, offers new insights into the behaviour of inflammatory conditions such as arthritis and cardiovascular disease which are known to be altered by the body clock”, added Dr Early.

Funded by Science Foundation Ireland, the research was undertaken in collaboration between RCSI, Trinity College Dublin and the Broad Institute in Boston, USA.

###

The paper, The Circadian Clock Protein BMAL1 Regulates IL-1β in Macrophages via NRF2, will be published on Monday, August 20, at 3 pm US ET/8pm GMT.

 

ChooseLife Thoughts : This is a good research area, Moreless used to repeatedly state ‘no proteins 4-6 hours before bed, no significant protein 6 hours ideally‘.

It rang true to me, he said if you eat significant protein before bed, your Autonomic Nervous System would potentially be held off from repair in key early sleep phases, as the digestion of proteins require more Oxygen than say vegetables/salad. If digestion is incomplete at the point of sleep, this triggers the endocrine system to focus blood flow there, rather than supplying electromagnetic force to tissue repair, recovering the adrenals (which happens between 11pm-1am it is believed), and other vital parasympathetic/R&R functions.

Coupling this information of Moreless, with Carey Reams suggestion, to just eat salad largely at dinner (with a good olive oil as dressing, he claimed this cleared the digestive tract well, creating a gelatine like substance to help avoid foods sticking, setting you up for the following day), light foods at dinner, which very likely shaped the base of Moreless thinking. They dovetail this research.

 

Brain pH Linked to Alzheimers

pH imbalance in brain cells may contribute to Alzheimer’s disease

Study identifies potential drug targets to reverse problem found in tiny organelles in astrocytes

Date:
August 2, 2018
Source:
Johns Hopkins Medicine
Summary:
Scientists say they have found new evidence in lab-grown mouse brain cells, called astrocytes, that one root of Alzheimer’s disease may be a simple imbalance in acid-alkaline — or pH — chemistry inside endosomes, the nutrient and chemical cargo shuttles in cells.

Full Article = sciencedaily.com

ChooseLife notes = Interestingly the pH of 6.21 was the average of those not affected, this is close to the tissue pH Carey Reams suggests we should be trying to be within (Below is a scheme showing the concept pH Range Of Acceptance):

Previously, I have seen some evidence, which Moreless cited, which showed that Fish demonstrate levels of Mercury tissue saturation, not chiefly based on the mercury levels of the water, but rather the pH. This strongly suggests that it is the pH which was the chief uptake driver of Mercury into tissue, not the levels of Mercury as you may assume (the studies showed a lake with lower pH and lower Mercury had Fish with higher levels of Mercury than another lake with higher pH and higher Mercury).

“Of interest, recent studies have shown that mercury levels in
water in a water-sediment partition system of high pH value were
higher than those in a comparable system of low pH (MATSUMURA et
al. 1972). Thus, we have the enigma of lower mercury content in
fish inhabiting waters of higher pH and comparably higher mercury
concentration. It appears that mercury concentration in water is
not the only factor controlling the amount of mercury in fish.” Full

This leads me to ponder, if similar is happening in our brains, with Aluminium starting to become more neuro-toxic as our pH drops? 

This similar principle, applied to dental Mercury, may explain why some do not suffer with amalgams, yet many feel they do? Perhaps those who suffer have generally lower saliva pH, which triggers the harmful effects, often believed to be from this element. 

(Alzheimers & Aluminium research = Link)